Insecticidal toxins from Bacillus thuringiensis subsp. kenyae: gene cloning and characterization and comparison with B. thuringiensis subsp. kurstaki CryIA(c) toxins.
作者： M A Von Tersch , H L Robbins , C S Jany , T B Johnson
DOI: 10.1128/AEM.57.2.349-358.1991
关键词:
摘要: Genes encoding insecticidal crystal proteins were cloned from three strains of Bacillus thuringiensis subsp. kenyae and two B. kurstaki. Characterization the toxin genes showed that they are most closely related to cryIA(c) The introduced into host strains, spectra activities each Cry protein determined for six pest lepidopteran insects. CryIA(c) as active kurstaki against Trichoplusia ni, Lymantria dispar, Heliothis zea, H. virescens but significantly less Plutella xylostella and, in some cases, Ostrinia nubilalis. sequence a gene was (GenBank M35524) compared with more than 99% identical show seven amino acid differences among predicted sequences 1,177 acids.
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