Lung injury and recovery in a murine model of unilateral acid aspiration: functional, biochemical, and morphologic characterization.

摘要: BACKGROUND Acid aspiration is a complication of general anesthesia. Most animal models developed to define its pathophysiology have focused on the acute (< or =24 h) phase injury. The authors describe model acid allowing study this type lung injury over time. METHODS instilled hydrochloric (0.1 m, 1.5 ml/kg) normal saline in right bronchus mice. Lung was evaluated at 6 h, 12 24 and 2 weeks by assessing arterial blood gases, respiratory system compliance, wet weight normalized body weight, myeloperoxidase activity, histology. Twelve hours after injury, computed tomography scan obtained. RESULTS In group, oxygen tension decreased (P < 0.05) whereas it recovered weeks; compliance lower both h 0.05). increased Myeloperoxidase activity peaked between h. Computed showed that almost 30% injured abnormally aerated. Although reduced, abnormalities were still present as confirmed fibrotic scar well evident histologic examination. CONCLUSION characterized murine regional long-term survival. Despite partial recovery, persisted, with evidence fibrosis reduction. This long-term, low-mortality seems suitable for assessment effects different therapies repair.