Pharmacokinetics of oxolinic acid in black tiger shrimp, Penaeus monodon Fabricius, and the effect of cooking on residues

摘要: AbstractThis study examined the pharmacokinetics andbioavailability of oxolinic acid (OA) in black tigershrimpPenaeus monodon Fabricius,inbrackishwater(salinity 10gL 1 )at28^291C, after intra-sinus (10mgkg ) and oral (50mgkg administration andalso investigated net changes OA residuesin shrimp cooking (boiling, baking andfrying).The haemolymph concentrations OAafterintra-sinusdosingwerebestdescribedbyatwo-com-partment open model.The distribution elimina-tion half-lives were 0.84 and17.7h respectively. Theapparent volume distributionata steadystate andthe total body clearance estimated to be2061mL kg 90.1mLkg h respectively.Thebioavailabilityof OAafteranoraladministrationwas 7.9%. The peak concentration,the time concentration elimination half-life administrationwere 4.20mgmL ,4h and19.8h Oxo-linic muscle shell levels increased amaximum (muscle1.76 mgg 8.17mgg )at 4h post then decreased withthe value 20.2 21.9hrespectively. Residual wasreduced by 20^30% each procedureexamined.Keywords: pharmacokinetics, oxolinicacid, Pena-eus monodon, tiger shrimp, bioavailability,cookingIntroductionIn Southeast Asia, intensive farming has de-veloped rapidly inthelast decade. Blacktiger shrimpPenaeusmonodon Fabriciusisaneconomicallyimpor-tant export inThailand, greater part pro-ductionis consumedbytheUSAandJapan.Recently,diseases caused byVibrio bacteria are a serious pro-blem causing economic damage theThai shrimpindustry.Toreduce diseaseproblems, awide range ofchemotherapeutics been used. use che-motherapeuticdrugs shrimpfarmingcan haveanimpact on aquatic environment potentiallycause health risks because drug residues theshrimpsoldforconsumption.Oneofthemajorenvir-onmental eiects using antibacterial agents isdevelopment bacterial resistance. Therefore, theproperuseofdrugsisstronglyrecommendedtoavoidsuch problems. Knowledge andresidue depletion is important minimize humanrisk associated with environ-mental impactof thedrugs.Currently,oxolinicacid(OA)isthemostcommonlyused agent Thai culture farms. phar-macokineticpro¢leofOAhaspreviouslybeenstudiedin rainbow trout Oncorhynchus mykiss (Walbaum)(Bj˛rklundBHustvedtSKlei-now, Jarboe, Shoemaker & Greenless 1994), Atlanticsalmon Salmo salar L. (Hustvedt, Salte Vassvik1991;Rogstad,EllingsenSMartinsen& Horsberg1995; Samuelsen, Ervik, Pursell Smith2000),channelcat¢shIctaluruspunctatusL.(Kleinow