T7 RNA polymerase bypass of large gaps on the template strand reveals a critical role of the nontemplate strand in elongation

摘要: We show that T7 RNA polymerase can efficiently transcribe DNA containing gaps from one to five bases in the template strand. Surprisingly, broken strands missing up 24 still be transcribed, although at reduced efficiency. The resulting transcripts contain full sequence with deleted for gapped region on These findings indicate end of a downstream strand brought into and transcribed as if it were part an intact polynucleotide chain by utilizing unpaired nontemplate This, well transcription strand, relies heavily upon non-template suggesting duplex DNA-binding site leading edge is required elongation templates. This work contributes substantially emerging picture important element complex.