Nonspecific, nested suppression PCR method for isolation of unknown flanking DNA.

作者: Richard Tamme , Esther Camp , R. Daniel Kortschak , Michael Lardelli

DOI: 10.2144/00285ST02

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摘要: We report the development of a simple, sensitive and robust two-step PCR method for isolation unknown sequences flanking characterized regions genomic DNA or cDNA. The requires 100...

参考文章(4)
Paul D. Siebert, Alex Chenchik, David E. Kellogg, Konstantin A. Lukyanov, Sergey A. Lukyanov, An improved PCR method for walking in uncloned genomic DNA Nucleic Acids Research. ,vol. 23, pp. 1087- 1088 ,(1995) , 10.1093/NAR/23.6.1087
M. S. Malo, K. Srivastava, J. M. Andresen, X. N. Chen, J. R. Korenberg, V. M. Ingram, Targeted gene walking by low stringency polymerase chain reaction: Assignment of a putative human brain sodium channel gene (SCN3A) to chromosome 2q24-31 Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 2975- 2979 ,(1994) , 10.1073/PNAS.91.8.2975
John Devereux, Paul Haeberli, Oliver Smithies, A comprehensive set of sequence analysis programs for the VAX Nucleic Acids Research. ,vol. 12, pp. 387- 395 ,(1984) , 10.1093/NAR/12.1PART1.387
S. Cheng, C. Fockler, W. M. Barnes, R. Higuchi, Effective amplification of long targets from cloned inserts and human genomic DNA. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 5695- 5699 ,(1994) , 10.1073/PNAS.91.12.5695