Schiff base adducts of hemoglobin. Modifications that inhibit erythrocyte sickling.
作者： R.H. Zaugg , J.A. Walder , I.M. Klotz
DOI: 10.1016/S0021-9258(19)75254-5
关键词:
摘要: Normal and sickle erythrocytes were exposed in vitro to millimolar concentrations of 31 different carbonyl compounds. Schiff base (imine) linkages formed with amino groups intracellular hemoglobin. Adducts isolated by gel electrofocusing could be dissociated dialysis. Aromatic aldehydes proved more reactive than aliphatic aldehydes, ketones unreactive. The influence various ring substituents on the reactivity aromatic was found conform closely traditional concepts regarding electronic steric effects. Several shown markedly increase oxygen affinity hemoglobins A S. In particular, 2,4-dihydroxybenzaldehyde o-vanillin, at 5 mM, produced 2- 3-fold reductions P50 (partial pressure half-saturation) hemoglobin whole blood. Since low degrees saturation promote erythrocyte sickling, compounds this type significantly inhibit sickling reduced partial pressures oxygen.
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