Molecular mechanism for the induction of "SOS" functions.

摘要: Publisher Summary This chapter describes the characterization of early molecular events that lead to induction prophages and SOS functions. The also obtains preliminary evidence specific oligodeoxynucleotide, such as d(A-G-G), inactivate phage (φ80 λ) repressors in permeabilized cells. strongly suggests oligodeoxynucleotide or single-stranded DNAs with base sequences are involved Permeabilized biologically active E.coli cells were obtained by plasmolysis incubation a reaction mixture allows protein synthesis. These become permeable low-molecular-weight proteins oligodeoxynucleotide. Using this system, one studied two types prophage induction. One is DNAse, micrococcal pancreatic DNase, other guanine-containing Prophage DNAse was dependent on functional recBC-DNase degradation DNA enzymes. On hand, independent did not require chromosomal DNA. There classes deoxy oligonucleotides effective deoxydinucleotides having sequence A(or G 1)-G; oligodeoxyguanylates 5’ phosphate chain length between 6 18.