The preservation of tissues for transplantation

摘要: This paper is a written version of lecture given during the celebration Professor Rudolf Klen’s 90th birthday. Dr. Klen played by far major part in introduction and development Tissue Banking Europe. His concept tissue bank envisaged storage all types cell, organ that physicians surgeons might need for treatment their patients. There has been much progress towards this goal, but still final objective remains elusive. review current position starts with recognition some tissues are required to comprise or include cells exhibit formal characteristics life if they function as grafts, whereas other do not. For tissues, preservation mechanical properties crucial: others it These considerations crucial design methods specific tissues: bone tendon skin can provide useful grafts absence living may even be true cardiac valves: requirement here remain intact. Simply freezing at around −80°C sufficient. In contrast, many cell systems, metabolizing organs require healthy function. Cryopreservation often an effective remedy isolated cells, example haemopoietic stem damaging effects formation ice sufficient rule out approach whole vascularised too. The mechanisms discussed, concluded site crystallization crucial. Cartilage hitherto recalcitrant, we have recently developed method permits stored liquid nitrogen temperatures without any recovery intact after storage. Thus, available help develop banking originally envisioned Klen.