Pulse schemes for the measurement of 3JC'C gamma and 3JNC gamma scalar couplings in 15N,13C uniformly labeled proteins.
作者： Robert Konrat , D.R. Muhandiram , N.A. Farrow , Lewis E. Kay
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摘要: Pulse sequences are presented for the measurement of3JC′Cγ and3JNCγ scalar couplings allCγ containing residues in15N,13C uniformly labeled proteins. The methodsdescribed based on quantitative J correlation spectroscopy pioneered byBax and co-workers [Bax et al. (1994) Methods Enzymol., 239, 79–105].The combination of 3JC′Cγ coupling constants allows theassignment discrete rotameric states about χ1 torsion angle in cases where such exist or, alternatively,facilitates establishment noncanonical χ1conformations or presence averaging. methods areapplied to a 1.5 mM sample staphylococcal nuclease.
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NMR of Proteins Macmillan Education UK. ,(1993) , 10.1007/978-1-349-12749-8
Ad Bax, Geerten W Vuister, Stephan Grzesiek, Frank Delaglio, Andy C Wang, Rolf Tschudin, Guang Zhu, Measurement of homo- and heteronuclear J couplings from quantitative J correlation Methods in Enzymology. ,vol. 239, pp. 79- 105 ,(1994) , 10.1016/S0076-6879(94)39004-5
Ad Bax, Stephan Grzesiek, Methodological advances in protein NMR Accounts of Chemical Research. ,vol. 26, pp. 131- 138 ,(1993) , 10.1007/978-1-349-12749-8_2
C. Griesinger, O. W. So/rensen, R. R. Ernst, Correlation of connected transitions by two‐dimensional NMR spectroscopy Journal of Chemical Physics. ,vol. 85, pp. 6837- 6852 ,(1986) , 10.1063/1.451421
Stephan Grzesiek, Ad Bax, The Importance of Not Saturating H2o in Protein NMR : application to Sensitivity Enhancement and Noe Measurements Journal of the American Chemical Society. ,vol. 115, pp. 12593- 12594 ,(1993) , 10.1021/JA00079A052
Steven L Patt, Single- and multiple-frequency-shifted laminar pulses Journal of Magnetic Resonance. ,vol. 96, pp. 94- 102 ,(1992) , 10.1016/0022-2364(92)90289-J
Martin Karplus, Contact Electron‐Spin Coupling of Nuclear Magnetic Moments The Journal of Chemical Physics. ,vol. 30, pp. 11- 15 ,(1959) , 10.1063/1.1729860
Geoffrey Bodenhausen, Richard R. Ernst, Alexander Wokaun, Alfred G. Redfield, Principles of nuclear magnetic resonance in one and two dimensions ,(1987)
Dominique Marion, Mitsuhiko Ikura, Ad Bax, Improved solvent suppression in one-and two-dimensional NMR spectra by convolution of time-domain data Journal of Magnetic Resonance. ,vol. 84, pp. 425- 430 ,(1989) , 10.1016/0022-2364(89)90391-0
H. Schwalbe, A. Rexroth, U. Eggenberger, T. Geppert, C. Griesinger, Measurement of C',C coupling constants in carbon-13 labeled proteins: a new method for the stereospecific assignment of .gamma.-methyl groups in valine residues Journal of the American Chemical Society. ,vol. 115, pp. 7878- 7879 ,(1993) , 10.1021/JA00070A044