Time-resolved proteomics of adenovirus infected cells

摘要: Viral infections cause large problems in the world and deeper understanding of disease mechanisms is needed. Here we present an analytical strategy to investigate host cell protein changes during human adenovirus type 2 (HAdV-C2 or Ad2) infection lung fibroblasts by stable isotope labelling amino acids culture (SILAC) nanoLC-MS/MS. This work focuses on early phase (6 12 h post-infection (hpi)) but data combined with previously published late (24 36 hpi) proteomics produce a time series covering complete infection. As many as 2169 proteins were quantitatively monitored from 6 hpi, while some time-specific. After applying different filter criteria, 2027 2150 quantified at hpi among them, 431 544 significantly altered two points. Pathway analysis showed that De novo purine pyrimidine biosynthesis, Glycolysis Cytoskeletal regulation Rho GTPase pathways activated inactivation Integrin signalling pathway started between hpi. Moreover, upstream regulator predicted MYC be RNA for genes controlled this transcription factor good correlation, which validated use prediction. Among identified phosphorylation sites, group related glycolysis cytoskeletal reorganization up-regulated The results show specific aspects how proteins, final products genetic information flow, are influenced Ad2 infection, would overlooked if only knowledge derived mRNA considered.