1H NMR and HPLC/DAD for Cannabis sativa L. chemotype distinction, extract profiling and specification

摘要: Abstract The medicinal use of different chemovars and extracts Cannabis sativa L. requires standardization beyond ∆9-tetrahydrocannabinol (THC) with complementing methods. We investigated the suitability 1 H NMR key signals for distinction four chemotypes measured in deuterated dimethylsulfoxide together two new validated HPLC/DAD methods used identification extract profiling based on main pattern cannabinoids other phenolics alongside assayed content THC, cannabidiol (CBD), cannabigerol (CBG) their acidic counterparts (THCA, CBDA, CBGA), cannabinol (CBN) cannflavin A B. Effects cell viability (MTT assay, HeLa) were tested. dominant cannabinoid pairs allowed chemotype recognition via assignment selective proton HPLC even cannabinoid-low from CBD CBG type. Substantial concentrations acids non-heated suggest consideration total values specifications herbal drugs extracts. Cannflavin A/B are extracted detected but always subordinated, while can be accumulated fractionation a wide fingerprint may equally serve as qualitative marker only. Cell reduction HeLa was more determined by than specific profile. Therefore analysis labeling THC 2–4 lead considered essential. analytical range compound groups summarized group ratio markers discussed regarding plant classification pharmaceutical specification.