DirecTag: accurate sequence tags from peptide MS/MS through statistical scoring.
作者： David L Tabb , Ze-Qiang Ma , Daniel B Martin , Amy-Joan L Ham , Matthew C Chambers
DOI: 10.1021/PR800154P
关键词:
摘要: In shotgun proteomics, tandem mass spectra of peptides are typically identified through database search algorithms such as Sequest. We have developed DirecTag, an open-source algorithm to infer partial sequence tags directly from observed fragment ions. This is unique in its implementation three separate scoring systems evaluate each tag on the basis peak intensity, m/z fidelity, and complementarity. data sets several types spectrometers, DirecTag reproducibly exceeded accuracy speed InsPecT GutenTag, two previously published for this purpose. The source code binaries available http://fenchurch.mc.vanderbilt.edu.
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