Ultraconserved non-coding DNA within insect phyla

摘要: Abstract Presence of ultra-conserved sequence elements in vertebrate enhancers suggest that transcription factor regulatory interactions are shared across phylogenetically diverse species. To date evidence for similarly conserved among evolutionarily distant insects such as flies, mosquitos, ants and bees, has been elusive. This study taken advantage the availability assembled genomic these to explore presence ultraconserved phylogenetic groups. investigate integrity fly sequences ~100 million years evolutionary divergence from fruitfly Drosophila melanogaster, we compared non-coding those Ceratitis capitata, Mediterranean fruit Musca domestica, domestic housefly. Using various alignment techniques, Blastn, Clustal, Blat, EvoPrinter Needle, show many blocks (CSBs) constitute cis-regulatory DNA, recognized by protocols, also Musca. We term species CSBs (uCSBs). The position uCSBs with respect flanking genes is conserved. results represent point interaction multiple trans-regulators whose functions divergent genera. Blastn alignments detect putative mosquitos Anopheles gambiae Culex pipiens Aedes aegypti. have identified bee Side side comparison ant EvoPrints identify between two taxa, well more poorly either one or other taxon but not both. Analysis dipterans, bees will lead a greater understanding their origin function sequences.