MiR-497 Suppresses YAP1 and Inhibits Tumor Growth in Non-Small Cell Lung Cancer
作者： Chongbiao Huang , Ruijue Ma , Jie Yue , Na Li , Zengxun Li
DOI: 10.1159/000430358
关键词:
摘要: Background/Aims: To investigate the expression, clinical significance and cellular effects of miR-497 in non-small cell lung cancer (NSCLC). Methods: NSCLC cells were transiently transfected with mimics or siRNA to up-regulate down-regulate expression. Quantitative real-time PCR (qRT-PCR) was used detect mRNA level miR-497. Luciferase assays, colony formation assays BrdU incorporation performed identify targets role cells. Finally, abundance analyzed a total 51 specimens. Results: The transcript levels significantly decreased tissue (25/30; 83.3%). Low tumor correlated advanced pT stage. Additionally, overall survival patients (n = 51, p 0.022). Overexpression inhibited proliferation down-regulation resulted elevated growth. Exogenous over-expression YAP1 partially eliminated miR-497-induced Conclusion: plays an important inhibiting by targeting YAP1. Our results suggest that is potential therapeutic target treating NSCLC.
core.ac.uk 
sci-hub.se 参考文章(33)
Cui Li, Wei Hong, Research status and funding trends of lung cancer biomarkers Journal of Thoracic Disease. ,vol. 5, pp. 698- 705 ,(2013) , 10.3978/J.ISSN.2072-1439.2013.10.10
Lei Zhang, Jianjun Qian, Yong Qiang, Hairong Huang, Changtian Wang, Demin Li, Biao Xu, Down-regulation of miR-4500 promoted non-small cell lung cancer growth. Cellular Physiology and Biochemistry. ,vol. 34, pp. 1166- 1174 ,(2014) , 10.1159/000366329
Veronica Balatti, Stefania Maniero, Manuela Ferracin, Angelo Veronese, Massimo Negrini, Gloria Ferrocci, Fernanda Martini, Mauro G. Tognon, MicroRNAs Dysregulation in Human Malignant Pleural Mesothelioma Journal of Thoracic Oncology. ,vol. 6, pp. 844- 851 ,(2011) , 10.1097/JTO.0B013E31820DB125
Xun Hu, Fan Zhang, Xiao-Rong Liu, Yun-Tao Wu, Yi-Ming Ni, Efficacy and potential microRNA mechanism for computed tomography-guided percutaneous radiofrequency ablation of primary lung cancer and lung metastasis from liver cancer. Cellular Physiology and Biochemistry. ,vol. 33, pp. 1261- 1271 ,(2014) , 10.1159/000358694
Jinliang Wang, Bo Yang, Lu Han, Xiaoyan Li, Haitao Tao, Sujie Zhang, Yi Hu, Demethylation of miR-9-3 and miR-193a genes suppresses proliferation and promotes apoptosis in non-small cell lung cancer cell lines. Cellular Physiology and Biochemistry. ,vol. 32, pp. 1707- 1719 ,(2013) , 10.1159/000356605
Lee P. Lim, Nelson C. Lau, Philip Garrett-Engele, Andrew Grimson, Janell M. Schelter, John Castle, David P. Bartel, Peter S. Linsley, Jason M. Johnson, Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs Nature. ,vol. 433, pp. 769- 773 ,(2005) , 10.1038/NATURE03315
Jian-Fu Chen, Elizabeth M Mandel, J Michael Thomson, Qiulian Wu, Thomas E Callis, Scott M Hammond, Frank L Conlon, Da-Zhi Wang, None, The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation Nature Genetics. ,vol. 38, pp. 228- 233 ,(2006) , 10.1038/NG1725
Jun Yu, Yuen Y. Cheng, Qian Tao, Kin F. Cheung, Cleo N.Y. Lam, Hua Geng, Lin–Wei Tian, Ying P. Wong, Joanna H.M. Tong, Jian–Ming Ying, Hongchuan Jin, Ka F. To, Francis K.L. Chan, Joseph J.Y. Sung, Methylation of protocadherin 10, a novel tumor suppressor, is associated with poor prognosis in patients with gastric cancer. Gastroenterology. ,vol. 136, pp. 640- 651 ,(2009) , 10.1053/J.GASTRO.2008.10.050
Yang Xia, Qiyou Chen, Zhaopeng Zhong, Caihua Xu, Chen Wu, Bin Liu, Yijiang Chen, Down-Regulation of MiR-30c Promotes the Invasion of Non-Small Cell Lung Cancer by Targeting MTA1 Cellular Physiology and Biochemistry. ,vol. 32, pp. 476- 485 ,(2013) , 10.1159/000354452
Junming Guo, Ying Miao, Bingxiu Xiao, Rong Huan, Zhen Jiang, Dan Meng, Yanjun Wang, Differential expression of microRNA species in human gastric cancer versus non-tumorous tissues. Journal of Gastroenterology and Hepatology. ,vol. 24, pp. 652- 657 ,(2009) , 10.1111/J.1440-1746.2008.05666.X