A novel loop-mediated isothermal amplification-based test for detecting Neospora caninum DNA.
作者： Andrea Estefanía Ramos , Marina Muñoz , Jesús Alfredo Cortés-Vecino , Paola Barato , Manuel Alfonso Patarroyo
DOI: 10.1186/S13071-017-2549-Y
关键词:
摘要: Neospora caninum is a cyst-forming, coccidian parasite which known to cause neurological disorders in dogs and abortion neonatal mortality cows other livestock. This study reports the development of loop-mediated isothermal amplification (LAMP) assay based on Nc-5 gene compares its efficacy for detecting DNA that semi-nested PCR test. Six primers were designed repeat region N. caninum. Specific LAMP led successful at 63 °C 30 min. The was highly specific (i.e. it did not reveal cross-reactivity with species) had low plasmid limit detection (1 fg), ten times higher than PCR. applicability evaluated using set naturally-infected samples (59 from canine faeces five bovine abortions). Thirty-nine percent (25/64) positive by 36% (23/64) However, much faster perform provides results optimized reaction conditions described this resulted sensitive, rapid technique DNA. Considering advantages DNA, further assays aimed testing usefulness wider range field are recommended.
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A Uggla, J L Carpenter, J P Dubey, C A Speer, M J Topper, Newly recognized fatal protozoan disease of dogs Javma-journal of The American Veterinary Medical Association. ,vol. 192, pp. 1269- 1285 ,(1988)
Robert C Edgar, MUSCLE: a multiple sequence alignment method with reduced time and space complexity BMC Bioinformatics. ,vol. 5, pp. 113- 113 ,(2004) , 10.1186/1471-2105-5-113
Lindsay Ds, Dubey Jp, Immunohistochemical diagnosis of Neospora caninum in tissue sections. American Journal of Veterinary Research. ,vol. 50, pp. 1981- ,(1989)
Chun-hua Gao, Dan Ding, Jun-yun Wang, Dietmar Steverding, Xia Wang, Yue-tao Yang, Feng Shi, Development of a LAMP assay for detection of Leishmania infantum infection in dogs using conjunctival swab samples Parasites & Vectors. ,vol. 8, pp. 370- 370 ,(2015) , 10.1186/S13071-015-0991-2
N Müller, V Zimmermann, B Hentrich, B Gottstein, Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay. Journal of Clinical Microbiology. ,vol. 34, pp. 2850- 2852 ,(1996) , 10.1128/JCM.34.11.2850-2852.1996
Patrice Francois, Manuela Tangomo, Jonathan Hibbs, Eve-Julie Bonetti, Catharina C. Boehme, Tsugunori Notomi, Mark D. Perkins, Jacques Schrenzel, Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications FEMS Immunology & Medical Microbiology. ,vol. 62, pp. 41- 48 ,(2011) , 10.1111/J.1574-695X.2011.00785.X
A Zintl, G McGrath, L O'Grady, J Fanning, K Downing, D Roche, M Casey, JS Gray, Changing epidemiology of the tick-borne bovine parasite, Babesia divergens Parasites & Vectors. ,vol. 7, pp. 1- 1 ,(2014) , 10.1186/1756-3305-7-S1-O8
Ji-guan Luo, Jun-wei Ge, Li-jie Tang, Xin-yuan Qiao, Yan-ping Jiang, Wen Cui, Min Liu, Yi-jing Li, Development of a loop-mediated isothermal amplification assay for rapid detection of bovine parvovirus. Journal of Virological Methods. ,vol. 191, pp. 155- 161 ,(2013) , 10.1016/J.JVIROMET.2012.05.002
Isaia Sotiriadou, Panagiotis Karanis, Evaluation of loop-mediated isothermal amplification for detection of Toxoplasma gondii in water samples and comparative findings by polymerase chain reaction and immunofluorescence test (IFT) Diagnostic Microbiology and Infectious Disease. ,vol. 62, pp. 357- 365 ,(2008) , 10.1016/J.DIAGMICROBIO.2008.07.009
Andy Alhassan, Oriel MM Thekisoe, Naoaki Yokoyama, Noboru Inoue, Makhosazana Y Motloang, Peter A Mbati, Hong Yin, Yoshinari Katayama, Toru Anzai, Chihiro Sugimoto, Ikuo Igarashi, None, Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis Veterinary Parasitology. ,vol. 143, pp. 155- 160 ,(2007) , 10.1016/J.VETPAR.2006.08.014