Transcriptional Pause Sites Delineate Stable Nucleosome-Associated Premature Polyadenylation Suppressed by U1 snRNP.
作者： Anthony C Chiu , Hiroshi I Suzuki , Xuebing Wu , Dig B Mahat , Andrea J Kriz
DOI: 10.1016/J.MOLCEL.2018.01.006
关键词:
摘要: Regulation of RNA polymerase II (Pol II) elongation is a critical step in gene regulation. Here, we report that U1 snRNP recognition and transcription pausing at stable nucleosomes are linked through premature polyadenylation signal (PAS) termination. By generating exosome conditional deletion mouse embryonic stem cells, identified large class polyadenylated short transcripts the sense direction destabilized by exosome. These PAS termination events enriched first few flanking CpG islands suppressed snRNP. Thus, promoter-proximal Pol consists two processes: TSS-proximal +1 nucleosome pausing, with coinciding latter. While factors NELF/DSIF only function former step, flavopiridol-sensitive mechanism(s) Myc modulate both steps. We propose near nucleosome-associated pause site represents common transcriptional checkpoint regulated recognition, stability, activity.
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