Immunoinformatic analysis of the SARS-CoV-2 envelope protein as a strategy to assess cross-protection against COVID-19.
作者： Bruno Tilocca , Alessio Soggiu , Maurizio Sanguinetti , Gabriele Babini , Flavio De Maio
DOI: 10.1016/J.MICINF.2020.05.013
关键词:
摘要: Envelope protein of coronaviruses is a structural existing in both monomeric and homo-pentameric form. It has been related to multitude roles including virus infection, replication, dissemination immune response stimulation. In the present study, we employed an immunoinformatic approach investigate major immunogenic domains SARS-CoV-2 envelope map them among homologue proteins with tropism for animal species that are closely inter-related human beings population all over world. Also, when not available, predicted folding mapped epitopes. sequences alignment provides evidence high sequence homology some investigated specimens; while mapping epitopes resulted interesting maintenance epitope localization also scoring lower score. line One-Health approach, our evidences provide molecular rationale potential role taxonomically conferring protection from infection identifying candidates development diagnostic tools prophylactic-oriented strategies.
unicz.it
elsevier.com参考文章(24)
Jaume Torres, Jifeng Wang, Krupakar Parthasarathy, Ding Xiang Liu, The transmembrane oligomers of coronavirus protein E Biophysical Journal. ,vol. 88, pp. 1283- 1290 ,(2005) , 10.1529/BIOPHYSJ.104.051730
Jose L. Nieto-Torres, Marta L. DeDiego, Enrique Álvarez, Jose M. Jiménez-Guardeño, Jose A. Regla-Nava, Mercedes Llorente, Leonor Kremer, Shen Shuo, Luis Enjuanes, Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein. Virology. ,vol. 415, pp. 69- 82 ,(2011) , 10.1016/J.VIROL.2011.03.029
Stephen F. Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, Yi-Kuo Yu, Protein database searches using compositionally adjusted substitution matrices FEBS Journal. ,vol. 272, pp. 5101- 5109 ,(2005) , 10.1111/J.1742-4658.2005.04945.X
Nicolas Guex, Manuel C. Peitsch, Torsten Schwede, Automated comparative protein structure modeling with SWISS-MODEL and Swiss-PdbViewer: a historical perspective. Electrophoresis. ,vol. 30, ,(2009) , 10.1002/ELPS.200900140
Ziad A Memish, Stanley Perlman, Maria D Van Kerkhove, Alimuddin Zumla, None, Middle East respiratory syndrome. The Lancet. ,vol. 386, pp. 995- 1007 ,(2015) , 10.1016/S0140-6736(15)60454-8
Jens Larsen, Ole Lund, Morten Nielsen, Improved method for predicting linear B-cell epitopes. Immunome Research. ,vol. 2, pp. 2- 2 ,(2006) , 10.1186/1745-7580-2-2
Linus Backert, Oliver Kohlbacher, Immunoinformatics and epitope prediction in the age of genomic medicine. Genome Medicine. ,vol. 7, pp. 119- 119 ,(2015) , 10.1186/S13073-015-0245-0
Namrata Tomar, Rajat K. De, Immunoinformatics: a brief review. Methods of Molecular Biology. ,vol. 1184, pp. 23- 55 ,(2014) , 10.1007/978-1-4939-1115-8_3
Jenny K. Stodola, Guillaume Dubois, Alain Le Coupanec, Marc Desforges, Pierre J. Talbot, The OC43 human coronavirus envelope protein is critical for infectious virus production and propagation in neuronal cells and is a determinant of neurovirulence and CNS pathology Virology. ,vol. 515, pp. 134- 149 ,(2018) , 10.1016/J.VIROL.2017.12.023
Wahyu Surya, Yan Li, Jaume Torres, Structural model of the SARS coronavirus E channel in LMPG micelles Biochimica et Biophysica Acta. ,vol. 1860, pp. 1309- 1317 ,(2018) , 10.1016/J.BBAMEM.2018.02.017