Effect of vasoactive intestinal peptide, somatostatin, and substance P on spontaneous IgE and IgG4 production in atopic patients.

摘要: The effect of vasoactive intestinal peptide (VIP), somatostatin (SOM), and substance P (SP) on spontaneous human IgE IgG4 production in atopic patients was studied. In cultures mononuclear cells (MNC), VIP inhibited both without affecting IgM, IgA, IgG1, IgG2, or IgG3 production. contrast, SOM only whereas SP other isotypes IgG subclasses. neuropeptides specific because each specifically blocked by a corresponding neuropeptide antagonist. To achieve the noted above, must be added at start culture. IFN-alpha IFN-gamma were found to inhibit prostaglandin E2 (PGE2) However, inhibition could not have been mediated IFN-alpha, IFN-gamma, PGE2 addition anti-IFN-alpha, anti-IFN-gamma, indomethacin, respectively, did reverse inhibition. contrast their effects MNC, affect either purified B cells; T monocytes had no this. effective inhibiting cultured together with monocytes. Depletion sIgE+ sIgG4+ resulted abrogation production, respectively. stimulation sIgE- IL-4 plus anti-CD 40 mAb induced which SOM, but SP, presence These results suggest that interaction cell- monocyte-dependent fashion. addition, also modulating switching