Relationships among Calcium-Dependent Protease, Cathepsins B and H, Meat Tenderness and the Response of Muscle to Aging

摘要: This study was conducted to compare the relative importance of Ca-dependent protease (CDP) and cathepsins B H meat tenderness changes in response postmortem cooler aging. Charolais bulls (n = 8) steers 7) were slaughtered at 15 mo age, total activities CDP-I (a with neutral pH optimum that requires micromolar amounts Ca for activity) (lysosomal proteases acidic optima) determined within 1 h. Shear-force values obtained after , 3, 6, 9 14 d Data pooled when analysis variance revealed no differences between steers. Initial shear force correlated (r -.71, P < . lo n activity (only seven animals sampled CDP-I), overall change (d 14) cathepsin .59, .05, 15). Most aging occurred 3 6 (41.6%), during this period related .44, .64, respectively, .05). Collectively, accounted 35 58% variation respectively. These data suggest helps establish initial 1) but are responsible tenderization occurs By manipulating live animal growth handling, it might be possible control through actions these enzyme systems. (