Ozone interaction with rodent lung: effect on sulfhydryls and sulfhydryl-containing enzyme activities

摘要: Exposure of rats or rat lung homogenates to 2 ppM O/sub 3/ reduced total, protein, and non-protein sulfhydryl fractions in a dose-related fashion. The fraction was more susceptible attack. Chronic exposure (0.8 ppM, 10 days) had no effect. Activities SH-containing enzymes, glutathione reductase (GR) glucose-6-phosphate dehydrogenase (G6PD), tissue decreased 15 30%, respectively, after 6 8 h 3/. In vitro (2 3 ppM) led reductions 20 67%. cytochrome C reductases 20% acute but increased chronic. SH-generating enzyme, G6PD, activity 32% little effect on GR activity. Lipid peroxidation (as possible mechanism for SH oxidation) not affected by vivo almost 100% vitro. G6PD signals hexose-monophosphate shunt pathway NADPH production. This is also active various other diseases (e.g., toxic liver injury).