Evaluation of clinical biomaterial surface effects on T lymphocyte activation

摘要: Previous in vitro studies our laboratory have shown that lymphocytes can influence macrophage adhesion and fusion on biomaterial surfaces. However, few evaluated how material adherent macrophages lymphocyte behavior, specifically T cells. In this study, we cultured human peripheral blood mononuclear cells from healthy donors three synthetic nonbiodegradable biomedical polymers: elasthane 80A (PEU), silicone rubber (SR), or polyethylene terephthalate (PET) tissue culture polystyrene (TCPS). Upregulation of cell surface activation markers (CD69 CD25), proliferation, interleukin-2 (IL-2) interferon-gamma (IFNgamma) concentrations were by flow cytometry, carboxy-fluorescein diacetate, succinimydyl ester (CFSE) incorporation, multiplex cytokine immunoassay, respectively, to assess activation. Following 3 7 days culture, CD4+ helper cultures any the groups did not express CD69 CD25 proliferation was present. IL-2 IFNgamma levels produced, but dependent donor. These data indicate are activated response clinically relevant biomaterials. The also suggest subsets exclusive source lymphokines, IFN-gamma, certain donors.