Flow cytometric sorting and RFLP analysis of phosphate accumulating bacteria in an enhanced biological phosphorus removal system.

摘要: Phosphate accumulating organisms (PAOs) stained with 4′,6-diamidino-2-phenylindol dihydrochloride (DAPI) at polyphosphate probing concentration were sorted from enhanced biological phosphorus removal (EBPR) sludge by flow cytometric sorting. All the genome DNA was extracted bacteria and 16S rDNA genes cloned. Cloned PCR-amplified analyzed restriction fragment length polymorphism (RFLP) analysis. Eighty eight clones RFLP patterns which appeared more than twice classified into seven groups. The most dominant group (Group 1) contained four accounted for 4.5% of total clones. Four groups (from Group 2 to 5) three 6 7 consisted two Sixty-eight gave unique patterns. By sequencing in groups, 1, 5 Rhodocyclus relatives (11%, 10/88). suggested be one responsible EBPR this sludge. Groups related b- or g-Proteobacteria. 4 belonged e-Proteobacteria. 3 green nonsulfur bacteria. Considering complex pattern existence not sequence analysis, system, together relatives, some other might also play a role as PAOs.