TGF-beta Induces Expression of Monocyte Chemoattractant JE/monocyte Chemoattractant Protein 1 via Transcriptional Factor AP-1 Induced by Protein Kinase in Osteoblastic Cells

摘要: In this work, we demonstrate the signal-transducing mechanism of TGF-beta 1 for gene expression monocyte chemoattractant JE/monocyte protein (MCP-1) in clonal osteoblastic MC3T3-E1 cells. 1-induced JE/MCP-1 cells was inhibited markedly by H-7 (1-(5-isoguinolinesulfonyl)-2-O-methylpiperazine-dihydrochloride) and staurosporine, potent inhibitors kinase. both early proto-oncogenes c-fos c-jun also staurosporine. Antisense oligonucleotides to genes significantly cytokine-induced expression. Curcumin, a specific inhibitor c-jun/AP-1, dose-dependent manner, though not affected. stimulated transcriptionally expression, stimulation curcumin. Curcumin-induced inhibition product evidenced an assay involving immunoprecipitation with antiserum chemotaxis. Curcumin AP-1 binding activity 12-tetradecanoyl phorbol-13-acetate-responsive element (TRE) cytokine-treated Furthermore, staurosporine TRE treated cytokine. These results that induces via transcriptional factor induced kinase