Improved method for identification and determination of .epsilon.-(.gamma.-glutamyl)lysine cross-link in protein using proteolytic digestion and derivatization with phenyl isothiocyanate followed by high-performance liquid chromatography separation

摘要: The cross-link was liberated by exhaustive proteolytic digestion and derivatized with PITC. derivatives were resolved HPLC. present method enables one to determine simultaneously protein-constituting amino acids ∈-(γ-glucyl)lysine thereby facilitates the monitoring of progress digestion. PTC-∈-(γ-glutamyl)lysine peak collected confirmed conversion a stable PTH form followed HCl hydrolysis