Method for generating induced pluripotent stem cells from keratinocytes derived from plucked hair follicles
作者： Joseph Itskovitz-Eldor , Atara Novak-Petraro , Ronit Shtrichman
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摘要: A method for generating induced pluripotent stem (iPS) cells from isolated hair follicles is disclosed. The comprises: a. culturing follicle keratinocytes on a layer of feeder cells, so as to generate colonies keratinocytes; b. detaching the detached c. infecting with virus comprising nucleic acid molecule encoding at least one dedifferentiation factor generated infected and d. in culture medium until iPS are formed, thereby cells. Populations uses also
lens.org参考文章(82)
Michael Krause, Transcription and translation. Methods in Cell Biology. ,vol. 48, pp. 483- 512 ,(1995) , 10.1016/S0091-679X(08)61400-4
H Mcconnell, Process for assaying for biologically active molecules ,(1974)
Cliff Tabin, Arlene Sharpe, Daniel Caput, Christopher Crum, Frank McKeon, Annie Yang, Ronen Schweitzer, Deqin Sun, Mourad Kaghad, Nancy Walker, Roderick T. Bronson, p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development Nature. ,vol. 398, pp. 714- 718 ,(1999) , 10.1038/19539
Laughton E. M. Miles, Universal reagent 2-site immunoradiometric assay using labelled anti (IgG) ,(1974)
Kirk R. Thomas, Mario R. Capecchi, Positive-negative selection methods and vectors ,(1993)
Julio E.Celis, Cell Biology: A Laboratory Handbook ,(1997)
Oshra Sedan, Katya Dolnikov, Naama Zeevi-Levin, Noa Leibovich, Michal Amit, Joseph Itskovitz-Eldor, Ofer Binah, 1,4,5‐Inositol Trisphosphate‐Operated Intracellular Ca2+ Stores and Angiotensin‐II/Endothelin‐1 Signaling Pathway Are Functional in Human Embryonic Stem Cell‐Derived Cardiomyocytes Stem Cells. ,vol. 26, pp. 3130- 3138 ,(2008) , 10.1634/STEMCELLS.2008-0777
David T. Denhardt, Raymond L. Rodriguez, Vectors : a survey of molecular cloning vectors and their uses Butterworths. ,(1988)
E John Coligan, Current protocols in immunology Greene Pub. Associates and Wiley-Interscience. ,(1991)
Jack R. Wands, Dominique Bellet, Multiepitopic immunometric assay ,(1987)