Structure and function of microbial communities in constructed wetlands - influence of environmental parameters and pesticides on denitrifying bacteria

摘要: This thesis addresses the interactions and relationships between natural aquatic bacterial communities, environmental parameters, anthropogenic chemicals denitrification pathway in habitat of agricultural constructed wetlands. The main aim was to gain fundamental knowledge drivers behind processes (i.e. nitrogen removal) wetlands, hence, structure function denitrifying community as efficient removal wetlands will decrease risk eutrophication freshwaters oceans. Programmes for restoring recreating areas have been initiated throughout world. Aquatic environments these are also exposed pollution from e.g. pesticides, fact, wetland with purpose reducing transport pesticides. However, little is known whether may fulfil both purposes simultaneously. Hence, maintain a high efficiency even during pesticide exposure? Both eubacterial communities were analysed, but focus has put on bacteria. Structural endpoints diversity heterogeneity analysed using molecular fingerprinting. Potential leucine incorporation growth) measured functional endpoints, when assessing effects exposure communities. These structural without any treatments well after exposure. results showed that (16S rRNA gene) (nirK, nirS nosZ) varied studied their influenced by parameters. enzyme gene higher than nirK nosZ, while had highest based richness. Exposure concentrations pesticides affected not (potential rate) community, however there few indications direct toxic effects. Using an endpoint activity growth quicker more sensitive method detect toxicity fungicides measuring potential denitrification, clear concentration-response easily generated could be standardized level assessments environments. (Less)