Near-infrared fluorescent probe with large stokes shift for detecting Human Neutrophil elastase in living cells.

作者: Yubing Zhou , Duolu Li , Wenda Zhang , Ting Ma

DOI: 10.1016/J.SAA.2021.119533

关键词:

摘要: Abstract Human Neutrophil elastase (HNE) plays a great role in immune responses and inflammation, is associated closely with lung cancer acute injury (ALI). Accurate detection of its activity imperative to understand biological function diagnosing the disease states through monitoring dynamic changes. Herein, we report new NIR fluorescent probe (F-1) large Stokes shift (182 nm). Probe F-1 featured high sensitivity (LOD ~ 5.6 ng/mL), good selectivity, low toxicity bright emission triggered by HNE. Moreover, was successfully applied as an indicator track HNE A549 cells. Thus, may be excellent tool for detecting enzymatic preclinical applications NE related diseases.

参考文章(33)
Maria Paola Giovannoni, Igor A. Schepetkin, Letizia Crocetti, Giovanna Ciciani, Agostino Cilibrizzi, Gabriella Guerrini, Andrei I. Khlebnikov, Mark T. Quinn, Claudia Vergelli, Cinnoline derivatives as human neutrophil elastase inhibitors Journal of Enzyme Inhibition and Medicinal Chemistry. ,vol. 31, pp. 628- 639 ,(2016) , 10.3109/14756366.2015.1057718
Lin Cheng, Qiang Zhao, Aptamer-capture based assays for human neutrophil elastase. Talanta. ,vol. 106, pp. 315- 320 ,(2013) , 10.1016/J.TALANTA.2012.11.016
Ka-Ho Leung, Hong-Zhang He, Victor Pui-Yan Ma, Hui Yang, Daniel Shiu-Hin Chan, Chung-Hang Leung, Dik-Lung Ma, A G-quadruplex-selective luminescent switch-on probe for the detection of sub-nanomolar human neutrophil elastase RSC Advances. ,vol. 3, pp. 1656- 1659 ,(2013) , 10.1039/C2RA21996D
Jing-Lin He, Zai-Sheng Wu, Song-Bai Zhang, Guo-Li Shen, Ru-Qin Yu, Fluorescence aptasensor based on competitive-binding for human neutrophil elastase detection Talanta. ,vol. 80, pp. 1264- 1268 ,(2010) , 10.1016/J.TALANTA.2009.09.019
Nicolaos Avlonitis, Manuelle Debunne, Tashfeen Aslam, Neil McDonald, Chris Haslett, Kevin Dhaliwal, Mark Bradley, Highly specific, multi-branched fluorescent reporters for analysis of human neutrophil elastase Organic and Biomolecular Chemistry. ,vol. 11, pp. 4414- 4418 ,(2013) , 10.1039/C3OB40212F
A. Jones, H. Elphick, E. Pettitt, M.L. Everard, G.S. Evans, Colistin stimulates the activity of neutrophil elastase and Pseudomonas aeruginosa elastase European Respiratory Journal. ,vol. 19, pp. 1136- 1141 ,(2002) , 10.1183/09031936.02.00230602
Alberto Mantovani, Marco A. Cassatella, Claudio Costantini, Sébastien Jaillon, Neutrophils in the activation and regulation of innate and adaptive immunity Nature Reviews Immunology. ,vol. 11, pp. 519- 531 ,(2011) , 10.1038/NRI3024
Qi Sun, Jun Li, Wan-Nian Liu, Qing-Jian Dong, Wen-Chao Yang, Guang-Fu Yang, Non-peptide-based fluorogenic small-molecule probe for elastase. Analytical Chemistry. ,vol. 85, pp. 11304- 11311 ,(2013) , 10.1021/AC402097G
Wenda Zhang, Feiyan Liu, Chao Zhang, Jian-Guang Luo, Jun Luo, Wenying Yu, Lingyi Kong, Near-Infrared Fluorescent Probe with Remarkable Large Stokes Shift and Favorable Water Solubility for Real-Time Tracking Leucine Aminopeptidase in Living Cells and In Vivo. Analytical Chemistry. ,vol. 89, pp. 12319- 12326 ,(2017) , 10.1021/ACS.ANALCHEM.7B03332
Shengjun Yang, Qingbin Zeng, Qianni Guo, Shizhen Chen, Hongbin Liu, Maili Liu, Michael T. McMahon, Xin Zhou, Detection and differentiation of Cys, Hcy and GSH mixtures by 19F NMR probe Talanta. ,vol. 184, pp. 513- 519 ,(2018) , 10.1016/J.TALANTA.2018.03.039