ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology

摘要: Growth and development of plants is ultimately driven by light energy captured through photosynthesis. ATP acts as universal cellular cofactor fuelling all life processes, including gene expression, metabolism, transport. Despite a mechanistic understanding biochemistry, dynamics in the living plant have been largely elusive. Here, we establish MgATP2- measurement using fluorescent protein biosensor ATeam1.03-nD/nA. We generate Arabidopsis sensor lines investigate vitro under conditions appropriate for cytosol. an assay fluxes isolated mitochondria, demonstrate that responds rapidly reliably to changes planta. A map seedling highlights different concentrations between tissues within individual cell types, such root hairs. Progression hypoxia reveals substantial plasticity homeostasis seedlings, demonstrating can be monitored plant.