The difference of glutathione antioxidant system in newly weaned and young mice liver and its involvement in isoline-induced hepatotoxicity
作者： Qing-Ning Liang , Yu-Chen Sheng , Ping Jiang , Li-Li Ji , Yu-Ye Xia
DOI: 10.1007/S00204-011-0664-7
关键词:
摘要: Cellular glutathione antioxidant system plays important roles in counteracting hepatotoxins-induced oxidative stress injury. The present study was designed to observe the differences of this newly weaned and young mice liver its involvement susceptibility isoline-induced Our results showed that reduced (GSH) amounts were higher than mice. Glutamate-cysteine ligase (GCL) activity due expression catalytic subunit GCL (GCLC) protein mRNA. However, activities reductase (GR), peroxidase (GPx), glutathione-S-transferase (GST) liver, which might be GR, GPx-1, GST-Pi proteins. Next, AST analysis histopathological evaluation demonstrated more severe injury induced by isoline. Furthermore, GSH GPx, GST all lower after treated with Depletion cellular d,l-buthionine-(S, R)-sulfoximine (BSO) aggravated cytotoxicity, while N-acetyl-l cysteine (NAC) ameliorated such cytotoxicity. inhibitors In conclusion, our between liver. Meanwhile, also revealed age-dependent isoline for first time, different responses
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