Measurement of glomerular blood flow in rabbits and rats: Erroneous findings with 15-µm microspheres

摘要: Measurement of glomerular blood flow in rabbits and rats: Erroneous findings with 15-µm microspheres. Intrarenal distribution was assessed rats the microsphere method. Collection individual glomeruli after silicone rubber arterial filling kidney permitted determination rate per glomerulus (GBF) from various cortical depth different vascular patterns: G 1 , most superficial; 4 true juxtamedullary (giving rise to vasa recta); 2 3 intermediate. In rabbits, GBFs were equal significantly higher than (G : 198 ± 21SEM, 101 18, 114 14, 197 13 nl/min). rats, GBF threefold that other 308 18; -G 108 16; 100 10). Mean size frequency microspheres found superficial deep rabbit, both differed significantly. Larger spheres preferentially species. This uneven sizes throughout cortex, as well unlikely filtration fractions are derived present values commonly reported for SNGFR, lead conclusion renal cortex does not reflect intracortical accurately rabbits. From study, no statement can be made concerning use technique dog. Mesure du debit sanguin glomerulaire chez le lapin et rat: Resultats errones obtenus par la des La intracorticale a ete etudiee rat Le glomerule determine pour quatre types de glomerules, preleves microdissection sur les reins injectes silastic voie arterielle: glomerules plus superficiels; juxtamedullaires (donnant naissance aux intermediaires. Chez lapin, est comparable dans significativement eleve que nl/mn). rat, trois fois celui autres 301 Les retrouvees superficiels ont un diametre moyen superieur profonds lapin. deux especes, l'etude histogrammes montre l'on retrouve une forte proportion grosses profonds. Ces resultats (repartition inegale differentes tailles situes differents niveaux cortex), ainsi valeurs peu vraisemblables fraction qui resultent ci-dessus (compte-tenu filtrations glomerulaires deja connues) permettent conclure ne reflete pas fidelement rat. Ce travail permet tirer conclusions en ce concerne l'application chien.