Sorghum Genome Sequencing by Methylation Filtration

摘要: Sorghum bicolor is a close relative of maize and staple crop in Africa much the developing world because its superior tolerance arid growth conditions. We have generated sequence from hypomethylated portion sorghum genome by applying methylation filtration (MF) technology. The evidence suggests that 96% genes been tagged, with an average coverage 65% across their length. Remarkably, this level gene discovery was accomplished after generating raw less than 300 megabases 735-megabase genome. MF preferentially captures exons introns, promoters, microRNAs, simple repeats, minimizes interspersed thus providing robust view functional parts set beneficial to research on also powerful resource for comparative genomics among grasses entire plant kingdom. Thousands hypothetical predictions rice Arabidopsis are supported dataset, genomic similarities highlight evolutionarily conserved regions will lead better understanding Arabidopsis.