Key Principles and Clinical Applications of “Next-Generation” DNA Sequencing

摘要: Demand for fast, inexpensive, and accurate DNA sequencing data has led to the birth dominance of a new generation technologies. So-called "next-generation" technologies enable rapid by massive amounts in parallel using diverse methodologies which overcome limitations Sanger methods used sequence first human genome. Despite opening frontiers genomics research, fundamental shift away from that next-generation created also left many unaware capabilities applications these technologies, especially those clinical realm. Moreover, brisk evolution flooded market with commercially available platforms, whose unique chemistries stand as another obstacle restricting potential sequencing. This review serves provide primer on researchers physician scientists. We an overview raise awareness among about power novel genomic tools. In addition, we discuss key principles comparison between existing near-term outline advantages disadvantages different platforms help choose appropriate platform their research interests.