Resolution of four large chromosomes in penicillin-producing filamentous fungi: the penicillin gene cluster is located on chromosome II (9.6 Mb) in Penicillium notatum and chromosome 1 (10.4 Mb) in Penicillium chrysogenum
作者： Francisco Fierro , Santiago Gutiérrez , Bruno Diez , Juan F. Martín
DOI: 10.1007/BF00279899
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摘要: Four chromosomes were resolved by pulsed field gel electrophoresis in Penicillium notatum (10.8, 9.6, 6.3 and 5.4 Mb size) five different strains of chrysogenum (10.4, 7.3 6.8 the wild type). Small differences size found between four P. strains. The penicillin gene cluster was localized hybridization with a pcbAB probe to chromosome II I all except deletion mutant npe10, which lacks this DNA region. pyrG AS-P-78 where hybridized 111. A major chromosomal rearrangement seems have occurred high producing strain. fast moving band observed gels corresponds mitochondrial DNA. total genome has been calculated as 32.1 34.1 for
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