Distribution of Telomeric DNA in Korean Native Chicken Chromosomes

摘要: . However, this hexamers can also occur at non-telomeric sites in some avians and vertbrate. This study was carried out to present the distribution of telomeric DNA sequences Korean Native Chicken chromosomes. The fluorescence situ hybridization technique using a probe performed metaphase spreads chicken early embryonic cells. Telomeric signals were detected ends chromosomes including macro-chromosomes microchromosomes. In chicken, surprisingly, chromosome 1 showed very distinct interstitial patterns which located two p-arm 1p11 1p23, one q-arm 1q32. number 2 3 displayed (ITS) long arms 2q24 3q32, respec-tively. pattern agreement with previously reported Gallus domesticus. relative amount each macrochromosomes ranged from 4.6% 16.3%. Distribution DNAs end much more than that almost chro-mosomes. ITS chromsomes implicate tandem fusions might have occurred during process karyotype evolution.(Key words : telomere, chromosomes, FISH, signal, chicken)To whom correspondence should be addressed shsohn@jinju.ac.kr(telomere)  !"#$%&'()*DNA+$,-./012345, 56DNA TTAGGG$78.9:;4 ?. @AB !CDEFG(HI(JDKLM 9N-O>HI(P, QR;ST?DNA #$&.UVMWXY4Z[\]QR^_N`9(\a?(Blackburn, 1991; Zakian, 1995; Shay, 1999; Weng Hodes, 2000). XbcQR^_deFfD(dg@A(mitotic clock):QR;dT?h50200 bp DHiV]5, j4KQR;FAOkFlmZ[nQRop(apotosis)4qPr8LQR^_ sa?(Harley, Wright et al., 1997; Faragher Kipling, 1998). %t01978uTetrahymenatelomeric sequenceNvw*xy(Blackburn Gall, 1978) 4z, 2009uBlackburn, Greider, Szostak4+{|4}(telomerase)x~%LL*^€KF*OK(‚?. Y4oƒ„ 525 kb(Harley 1990), T }50200 kb(Kipling Cooke, i†15 kb, ‡18 kb DH(Kozik 1998)ˆ‰Š*‹, ŒB0.5 kb20 Mb?Ž%W ‘N’G)*“”=>?(Delany •, ŒTTAGGG sequence\–—34% DHoƒ0.3%+˜10™4Kš4N›45>?(Delany 2003). q8L* !"#$%Ra)Fœ ()*, qFž˜RGBtelomeric sequence !"#$%lPŸ XbP1i„ , ¢£ŒF¤