Laser-excited Raman spectroscopy of biomolecules: I. Native lysozyme and its constituent amino acids☆

摘要: Laser-excited Raman spectra of a simple native protein, lysozyme, in aqueous solution are reported and partially interpreted with the help its constituent amino acids. spectroscopy should be useful providing direct evidence concerning presence number disulfide cross-links proteins, may also studying local conformation CSSC group. The aromatic side-groups phenylalanine, tryptophan tyrosine give rise to very intense sharp lines. These lines not sensitive changes or state aggregation. peptide CONH group gives two characteristic near 1660 (amide I) 1260 cm−1 III). They appear potentially assessment conformational caused by denaturation. Lines region from 800 1150 arising CC CN stretching vibrations expected conformation-dependent.