Transcription of the trp operon in Lactococcus lactis is controlled by antitermination in the leader region.
作者： H. Frenkiel , J. Bardowski , S. D. Ehrlich , A. Chopin
DOI: 10.1099/00221287-144-8-2103
关键词:
摘要: The regulatory functions of the leader region preceding Lactococcus lactis trp operon have been studied by mutagenesis analysis. This presents striking similarity to ‘T-box’ leaders found upstream many Gram-positive aminoacyl-tRNA synthetase genes and some amino acid biosynthesis operons, which are controlled antitermination through interaction transcript with cognate uncharged tRNA. A L. also contains a series (G/U)AG repeats which, in Bacillus, involved binding RNA-binding protein (TRAP) controls transcription. screen was developed for isolation mutants affected region. All spontaneous contained deletions; point mutations were only obtained after UV-induced mutagenesis. putative transcription terminator operon, demonstrating that is indeed antitermination.
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