MiR-138-5p suppresses lung adenocarcinoma cell epithelial-mesenchymal transition, proliferation and metastasis by targeting ZEB2.

摘要: Abstract Background MiR-138-5p is regarded as a tumour suppressor in many cancers. Transforming growth factor beta (TGF-β) often acts tumor promotor at the late stages of human However, function miR-138-5p on lung adenocarcinoma cells induced by TGF-β remains to be further confirmed. Methods RT-qPCR was used detect expression tissues, adjacent normal and relative cell lines. When A549 H1299 were transfected with negative control (NC), mimics inhibitor lipofectamine3000 treated or without TGF-β1, detected Immunofluorescence, Western blotting (WB), counting Kit-8 (CCK8), colony formation, EdU, Wound-healing Transwell assays. The relation between zinc finger E-box-binding homeobox 2 (ZEB2) RT-qPCR, WB, Luciferase reporter ZEB2 knocked down, CCK8 Results decreased tissues overexpression suppressed EMT, proliferation metastasis H1299. verified direct target miR-138-5p. Downregulation cell, which could reversed inhibitor. Conclusions inhibits epithelial-mesenchymal transition, through targeting ZEB2.