Base-unpaired regions in supercoiled replicative form DNA of coliphage M13.
作者： S Dasgupta , D P Allison , C E Snyder , S Mitra
DOI: 10.1016/S0021-9258(17)40112-8
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摘要: Superhelical covalently closed circular replicative form DNA (RF I) of coliphage M13 appears as a relaxed molecule that has base-unpaired region in the bubble (100 to 200 base pairs long) seen electron micrographs when spread presence formaldehyde and formamide or after pretreatment with glyoxal. S1 endonuclease, specific for single-stranded DNA, converts superhelical RF I but not nonsuperhelical significant extent, into unit-length linear molecules by sequential nicking two strands. The locations nuclease-susceptible sites glyoxal-fixed regions were both related five A-T-rich DNA. While nuclease does show preference any these sites, bubbles occur predominantly at major
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