Molecular characterization and evidencing of the porcine CRH gene as a functional-positional candidate for growth and body composition.
作者： Eduard Muráni , Mária Murániová , Siriluck Ponsuksili , Karl Schellander , Klaus Wimmers
DOI: 10.1016/J.BBRC.2006.01.143
关键词:
摘要: Abstract Corticotropin-releasing hormone (CRH), a major regulator of neuroendocrine response to stress, is involved in the control energy balance and thus may affect body composition growth. The porcine CRH (pCRH) gene was studied as comparative-positional candidate for QTL longissimus muscle area, average backfat thickness, carcass length, daily gain on test chromosome 4. Sequence complete transcriptional unit pCRH spanning 2068 bp determined along with 582 bp 5′-flanking region. Cross-species sequence comparison revealed number potential regulatory regions including an intronic evolutionary conserved region adjacent CpG island that cell-type specific expression gene. A SNP exon 2 (c.+83G > A) leading non-conservative amino acid exchange (p.28Arg > Gln) prohormone identified segregating DUMI resource population. Linkage association analysis based this all four traits falls peak area c.+83G > A shows highly significant additive effect (p T) occurred motif. knowledge DNA-variation will facilitate follow-up studies necessary provide definite genetic evidence
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