Kinetic analysis of decreased sperm fertilizing ability by fluorides and fluoroaluminates: a tool for analyzing the effect of environmental substances on biological events

摘要: Fluorides and fluoroaluminates decrease mouse sperm fertilizing potential by modifying the process of preparation for fertilization, so-called capacitation, followed acrosome reaction (AR). Capacitation was monitored protein tyrosine phosphorylation (pTyr), AR induced consequently. The aim this study to apply kinetic analysis previously obtained dependences pTyr at capacitation times, propose a mathematical theory mechanism when maturation ability is amended external stimuli. experimental input data, obtained, are consistent with proposed results show that runs as two subsequent first-order steps. Firstly, an unstable intermediate formed then gradually decomposes. time corresponding maximal production probably most suitable obtaining fertilize egg. presented calculations indicate application can serve tool predict or confirm course biological events modified factors, therefore shall be interest broad scientific audience.