Dual Action of NAMI-A in Inhibition of Solid Tumor Metastasis Selective Targeting of Metastatic Cells and Binding to Collagen

摘要: NAMI-A is a ruthenium complex endowed with selective effect on lung metastases of solid metastasizing tumors. The aim this study to provide evidence that NAMI-A's based the sensitivity metastasis cell, as compared other tumor cells, and show lungs represent privileged site for antimetastatic effects. transplantation Lewis carcinoma harvested from primary mice treated 35 mg/kg/day six consecutive days, dose active metastases, shows no change in take growth but significant reduction formation spontaneous metastases. Transmission electron microscopy examination kidney selectively bind collagen extracellular matrix also type IV basement membrane glomeruli. half lifetime elimination longer than liver, kidney, tumor. bound cells shown vitro by an invasion test, using modified Boyden chamber Matrigel, it inhibits metallo-proteinases MMP-2 MMP-9 at micromolar concentrations, zimography test. These data significantly affect metastatic ability. Binding allows exert its activity during dissemination particularly lungs. stress wide spectrum daily doses treatment schedules which against