SINGLE NUCLEOTIDE RESOLUTION OF STEROL REGULATORY REGION IN PROMOTER FOR 3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE

摘要: Sterol-dependent regulation of the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase promoter was previously localized to a 42-base pair region containing an octamer sequence, referred as sterol regulatory element (SRE-1). similar motif is found in DNA that required for sterol-dependent HMG-CoA synthase and low density lipoprotein receptor genes. Single nucleotide substitution analyses promoters confirmed SRE-1 important motif. In current studies, series single mutations were introduced into transfected Chinese hamster ovary cells. RNA produced by each mutant then measured presence or absence sterols. Thirty-seven independent analyzed, two separate domains identified being critical. One essential spread over 10 bases contained half SRE-1; however, other not regulation. The second spanned four contiguous bases. These critical elements are separated from three nonessential results interpreted suggest gene transcription sterols requires additional possibly factors those promoters.