C1q: Rapid Purification Method for Preparation of Monospecific Antisera and for Biochemical Studies

摘要: C1q has been purified using precipitation in the presence of chelating agents at low ionic strength. The three times precipitated was highly as shown by immunoelectrophoresis and analytic acrylamide disc electrophoresis. On immunoelectrophoresis, showed a single line slow γ-region against potent anti-whole human antiserum, hemolytic activity latex agglutination coincided with this region. final product but did not show any detectable C1r or C1s activity. yield these methods is about 40 to 60% thus 5 mg can be obtained from 100 ml serum. 3.75% preparative gels containing sodium dodecyl sulfate yields monospecific antisera when injected into rabbits. These agglutinate EAC1q cells EA. molecular weight estimated its relative mobility on 387,600 ± 10,790, breakdown smaller subunits demonstrated urea.