miR-1207-5p and miR-1266 suppress gastric cancer growth and invasion by targeting telomerase reverse transcriptase

摘要: hTERT is the catalytic subunit of telomerase complex. Elevated expression associated with expansion and metastasis gastric tumor. In this study, we aimed to identify novel tumor suppressor miRNAs that restrain expression. We began our screen for hTERT-targeting a miRNA microarray. candidates were further filtered by bioinformatic analysis, general pattern in different cell lines, gain-of-function effects on protein potential these suppress 3′ untranslated region (3′UTR) luciferase activity. The clinical relevance two (miR-1207-5p miR-1266) was evaluated real-time RT-PCR. growth, cycle invasion cancer cells measured CCK-8, flow cytometry transwell assays. Finally, ability transplanted tumors also investigated. Fourteen identified using combination bioinformatics microarray analysis. Of fourteen miRNAs, nine expressed at significantly lower levels hTERT-positive lines compared hTERT-negative five could downregulate Only miR-1207-5p miR-1266 interacted UTR decreased tissues. These inhibited growth vitro vivo. Altogether, determined be suppressors cancer, delivery represents therapeutic strategy treatment.