Transferable plasmid mediating resistance to multiple antimicrobial agents in Klebsiella pneumoniae isolates in Greece

作者: I. Galani , E. Xirouchaki , K. Kanellakopoulou , G. Petrikkos , H. Giamarellou

DOI: 10.1046/J.1469-0691.2002.00391.X

关键词:

摘要: Abstract Objective To investigate the underlying resistance mechanisms in 10 Klebsiella pneumoniae isolates. Methods Ten K. strains according to distinct bacteriocin typing and REP-PCR, were examined for their plasmid content, ability transfer aminoglycosides third-generation cephalosporins, production of aminoglycoside-modifying enzymes β-lactamases. Results Transfer above-mentioned antibiotics as well co-trimoxazole tetracycline Escherichia coli strain RC 85 at a frequency 5–10 6 was achieved all by conjugation. Similar harbor self-transferable multiresistant (80 kb) with similar Eco RI Hind III restriction patterns. This encodes an extended-spectrum β-lactamase which confers high-level cephalosporins aztreonam. It produces SHV-5 β-lactamase, demonstrated isoelectric focusing DNA sequencing. Aminoglycoside co-transferred, AAC(6′)-I, mediating gentamicin, tobramycin, netilmicin amikacin, AAC(3)-I, gentamicin sisomycin, encoded isolates transconjugants, while APH(3′)-I, kanamycin neomycin, seven strains. Conclusions appears that transferable encoding causing unusually high ceftazidime aztreonam, combination AAC(6′)-I + AAC(3)-I acetylating causing, also clinically available aminoglycosides, is established Greece.

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