Peptides derived from the prohormone proNPQ/spexin are potent central modulators of cardiovascular and renal function and nociception

摘要: Computational methods have led two groups to predict the endogenous presence of a highly conserved, amidated, 14-aa neuropeptide called either spexin or NPQ. NPQ/spexin is part larger prohormone that contains 3 sets RR residues, suggesting it could yield more than one bioactive peptide; however, no in vivo activity has been demonstrated for any peptide processed from this precursor. Here we demonstrate biological peptides present within proNPQ/spexin. (NWTPQAMLYLKGAQ-NH(2)) and NPQ 53-70 (FISDQSRRKDLSDRPLPE) differing renal cardiovascular effects when administered intracerebroventricularly intravenously into rats. Intracerebroventricular injection produced 13 ± 2 mmHg increase mean arterial pressure, 38 8 bpm decrease heart rate, profound urine flow rate. administration 26 9 rate with change marked Intraventricular also antinociceptive warm water tail withdrawal assay mice (ED(50)<30 10 nmol 53-70, respectively). We conclude newly identified derived precursor contribute CNS-mediated control blood pressure salt balance modulate nociceptive responses.