[42] Desalting of peptides

摘要: Publisher Summary This chapter discusses the various methods used for desalting of peptides. The described have limitations that restrict their application and are only removal sodium or potassium salts. A column Dowex 50-X2 is prepared from equilibrated resin. It suitable quantities peptide up to 20 micromoles. solution be desalted diluted with water until concentration cations 0.1 M less. Desalting l-X2 method those peptides which cannot 50-X2. Resin as supplied by manufacturer in 100–150 mesh range particle size without pretreatment. procedure amberlite IRC-50 distributed under designation CG-50, a pulverized material dust must removed decantation. such reduced below 0.2 N. An ion-exchange general utility all but strongly acidic relies on affinity sulfonated polystyrene resins at low pH.