In vitro cell culture in media containing beta-alanyl-taurine or carbobenzoxy beta-alanyl-taurine
作者： Galen D. Knight , Terence J. Scallen
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摘要: Compounds termed "vitaletheine modulators" which include beta-alanyl-taurine and carbobenzoxy are synthesized added to culture media for in vitro of cells such as mammalian or plant cells. The compounds support cell vitality, provide increased cellular life span, bioproductivity, improved function, adaption resistant culturing. Carbobenzoxy is produced by coupling β-alanine, has it's terminal amine protected with a (CBZ) group, N-hydroxysuccinimide produce an active ester two the esters cystamine CBZ-protected β-alethine having internal disulfide bond, isolating purifying β-alethine, reacting iodine oxidize bond obtain beta-alanyl-taurine. Beta-alanyl-taurine same procedure except that after oxidizing iodine, irradiated UV radiation remove CBZ-protecting group.
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