Concurrent ethene generation and growth of Dehalococcoides containing vinyl chloride reductive dehalogenase genes during an enhanced reductive dechlorination field demonstration.

摘要: Dehalococcoides bacteria that produce catabolic vinyl chloride (VC) reductive dehalogenase enzymes have been implicated as a requirement for successful biological dechlorination of VC to ethene in groundwater systems. Therefore, the functional genes reductase (e.g., vcrA) may be important biomarkers predicting and monitoring performance bioremediation systems treating chloroethenes via enhanced (ERD). As part an ERD field demonstration, 45 samples were analyzed vcrA using quantitative PCR. The demonstration delivered lactate continuously recirculation over 201 days aquifer contaminated with cis-1,2-dichloroethene (cDCE, ∼150 μM) (∼80 μM). Ethene (∼4 containing (average concentration 4 × 103 gene copies L−1) detected priori plot; however, materials bench treatability test able dechlorinate cDCE only 4-m...