Amlodipine ameliorates endothelial dysfunction in mesenteric arteries from spontaneously hypertensive rats.

摘要: 1. Endothelial dysfunction plays a critical role in the development and progression or pathogenesis of hypertension. Amlodipine, calcium channel blocker, is an effective antihypertensive agent. We investigated effects amlodipine on endothelial mesenteric arteries from spontaneously hypertensive rats (SHR). 2. Eight-week-old SHR were treated with (10 mg/kg per day) for 8 weeks. Control Wistar-Kyoto (WKY) saline. Systolic blood pressure (SBP) was measured by tail-cuff method. Isometric tension changes isolated arterial rings recorded continuously myograph system. Serum contents malondialdehyde (MDA) total nitrate/nitrite (NO(x) ) determined. Vascular superoxide anion production analysed dihydroethidium (DHE) fluorescence. 3. The contractile responses to KCl phenylephrine greater untreated than WKY. Acetylcholine (ACh)-induced relaxation significantly impaired SHR. Amlodipine treatment reduced contractions improved ACh. In WKY, ACh inhibited N(G) -nitro-l-arginine methyl ester (l-NAME) not changed ascorbic acid. SHR, response unaffected l-NAME, whereas it restored inhibitory effect l-NAME ACh-induced relaxation, but acid no longer exerted its facilitating effect. prevented rise SBP ameliorated abnormalities serum MDA NO DHE assay showed increased intravascular generation which abrogated amlodipine. 4. Treatment resulted amelioration anti-oxidant activity improvement availability.